Polyamine content of nucleated and enucleated Escherichia coli cells
نویسندگان
چکیده
منابع مشابه
Polyamine content of nucleated and enucleated Escherichia coli cells.
There is currently considerable interest in the physiological function(s) of polyamines. Investigations have shown many in vitro interactions of polyamines with various cellular components, particularly deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and ribosomes; the in vivo role(s) of polyamines is still unclear (H. Tabor and C. W. Tabor, Pharmacol. Rev. 16:245, 1964). This is owing lar...
متن کاملAdjustment of polyamine contents in Escherichia coli.
Adjustment of polyamine contents in Escherichia coli was studied with strains of Escherichia coli producing normal (DR112) and excessive amounts of ornithine decarboxylase [DR112(pODC)] or S-adenosylmethionine decarboxylase [DR112(pSAMDC)]. Although DR112(pODC) produced approximately 70 times more ornithine decarboxylase than DR112 did, the amounts of polyamines in the cells of both strains did...
متن کاملThe effect of isolation conditions on the polyamine content of Escherichia coli ribosomes.
The polyamine content of isolated E. coli ribosomes is dependent on the nature and concentration of the ions in the medium used for their preparation. When ribosomes are suspended in medium containing amines, the external amines are taken up by the ribosomes. When large amounts of amines are taken up by the ribosomes, part of the original ribosomal amines are displaced into the medium. Magnesiu...
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During the course of a urinary tract infection, substantial levels of nitric oxide and reactive nitrogen intermediates are generated. We have found that many uropathogenic strains of Escherichia coli display far greater resistance to nitrosative stress than the K-12 reference strain MG1655. By selecting and screening for uropathogenic E. coli transposon mutants that are unable to grow in the pr...
متن کاملApparently unidirectional polyamine transport by proton motive force in polyamine-deficient Escherichia coli.
A transport system for polyamines was studied with both intact cells and membrane vesicles of an Escherichia coli polyamine-deficient mutant. Polyamine uptake by intact cells and membrane vesicles was inhibited by various protonophores, and polyamines accumulated in membrane vesicles when D-lactate was added as an energy source or when a membrane potential was imposed artificially by the additi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.95.5.1966-1967.1968